Composite

Part:BBa_K912022:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23110(medium) promoter/RBS+ E. coli phytase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 729
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1254


Design Notes

None besides primer design for pcring out the e. coli phytase from the genome


Source

Constitutive promoter: registry E. coli Phytase: genomic sequence

References